1899 U.S. National Championships – Women's singles

Marion Jones won the singles tennis title of the 1899 U.S. Women's National Singles Championship by defeating Maud Banks 6–1, 6–1, 7–5 in the final of the All Comers' competition. The reigning champion Juliette Atkinson did not defend her title and therefore no challenge round was held. The event was played on outdoor grass courts and held at the Philadelphia Cricket Club in Wissahickon Heights, Chestnut Hill, Philadelphia from June 21 through June 24, 1899.