2010 US Open – Women's singles

Defending champion Kim Clijsters defeated Vera Zvonareva in the final, 6–2, 6–1 to win the women's singles tennis title at the 2010 US Open. It was her third US Open title and third major title overall.

World No. 1 and three-time champion Serena Williams withdrew from the tournament due to a foot surgery sustained from an exhibition match. Caroline Wozniacki was in contention to gain the top ranking by winning the title, but lost to Zvonareva in the semifinals.

This was the final major appearance for former world No. 3 Elena Dementieva; she was defeated in the fourth round by Samantha Stosur.

Seeds
• Caroline Wozniacki (semifinals)

• 🇧🇪 Kim Clijsters (champion)

• 🇺🇸 Venus Williams (semifinals)

• 🇷🇸 Jelena Janković (third round)

• 🇦🇺 Samantha Stosur (quarterfinals)

• 🇮🇹 Francesca Schiavone (quarterfinals)

• 🇷🇺 Vera Zvonareva (final)

• 🇨🇳 Li Na (first round)

• 🇵🇱 Agnieszka Radwańska (second round)

• 🇧🇾 Victoria Azarenka (second round, retired due to concussion)

• 🇷🇺 Svetlana Kuznetsova (fourth round)

• 🇷🇺 Elena Dementieva (fourth round)

• 🇫🇷 Marion Bartoli (second round)

• 🇷🇺 Maria Sharapova (fourth round)

• 🇧🇪 Yanina Wickmayer (fourth round)

• 🇮🇱 Shahar Pe'er (fourth round)

• 🇷🇺 Nadia Petrova (first round)

• 🇫🇷 Aravane Rezaï (second round)

• 🇮🇹 Flavia Pennetta (third round)

• 🇷🇺 Anastasia Pavlyuchenkova (fourth round)

• 🇨🇳 Zheng Jie (second round)

• 🇪🇸 María José Martínez Sánchez (second round)

• 🇷🇺 Maria Kirilenko (third round)

• 🇸🇰 Daniela Hantuchová (third round)

• 🇷🇴 Alexandra Dulgheru (third round)

• 🇨🇿 Lucie Šafářová (first round)

• 🇨🇿 Petra Kvitová (third round)

• 🇷🇺 Alisa Kleybanova (second round)

• 🇺🇦 Alona Bondarenko (third round)

• 🇰🇿 Yaroslava Shvedova (first round)

• 🇪🇪 Kaia Kanepi (quarterfinals)

• 🇧🇬 Tsvetana Pironkova (second round)