2001 US Open – Women's singles

Defending champion Venus Williams defeated her sister Serena Williams in the final, 6–2, 6–4 to win the women's singles tennis title at the 2001 US Open. It was her fourth major singles title, and she did not lose a set during the tournament. The championship match was the first of nine major finals contested by the sisters, and the first US Open women's singles final to take place during American television prime time.

Seeds
• # Martina Hingis (semifinals)

• # 🇺🇸 Jennifer Capriati (semifinals)

• # 🇺🇸 Lindsay Davenport (quarterfinals)

• # 🇺🇸 Venus Williams (champion)

• # 🇧🇪 Kim Clijsters (quarterfinals)

• # 🇧🇪 Justine Henin (fourth round)

• # 🇺🇸 Monica Seles (fourth round)

• # 🇫🇷 Amélie Mauresmo (quarterfinals)

• # 🇫🇷 Nathalie Tauziat (fourth round)

• # 🇺🇸 Serena Williams (final)

• # 🇷🇺 Elena Dementieva (fourth round)

• # 🇺🇸 Meghann Shaughnessy (third round)

• # Amanda Coetzer (first round)

• # Jelena Dokić (fourth round)

• # 🇧🇬 Magdalena Maleeva (second round)

• # 🇮🇹 Silvia Farina Elia (first round)

• # 🇩🇪 Anke Huber (third round)

• # 🇫🇷 Sandrine Testud (fourth round)

• # 🇦🇹 Barbara Schett (fourth round)

• # 🇪🇸 Arantxa Sánchez Vicario (third round)

• # 🇷🇺 Elena Likhovtseva (fourth round)

• # 🇺🇿 Iroda Tulyaganova (second round)

• # 🇪🇸 Magüi Serna (first round)

• # 🇦🇷 Paola Suárez (first round)

• # 🇸🇰 Henrieta Nagyová (third round)

• # 🇹🇭 Tamarine Tanasugarn (first round)

• # 🇪🇸 Ángeles Montolio (third round)

• # 🇺🇸 Chanda Rubin (third round)

• # 🇺🇸 Amy Frazier (first round)

• # 🇺🇸 Lisa Raymond (third round)

• # 🇪🇸 Cristina Torrens Valero (first round)

• # 🇮🇹 Francesca Schiavone (first round)