2012 Citi Open – Women's singles

Nadia Petrova was the defending champion, but opted to play at the London Summer Olympics instead.

Magdaléna Rybáriková won the title, defeating top seed Anastasia Pavlyuchenkova 6–1, 6–1, in the final.

Seeds
• # 🇷🇺 Anastasia Pavlyuchenkova (final)

• # Chanelle Scheepers (first round)

• # 🇺🇸 Sloane Stephens (semifinals)

• # 🇺🇸 Vania King (semifinals)

• # 🇨🇿 Barbora Záhlavová-Strýcová (first round)

• # 🇨🇿 Iveta Benešová (first round)

• # 🇺🇸 Coco Vandeweghe (quarterfinals)

• # 🇧🇾 Olga Govortsova (second round)

Seeds
• # 🇫🇷 Aravane Rezaï (qualified)

• # Michelle Larcher de Brito (qualified)

• # 🇸🇰 Jana Čepelová (qualified)

• # 🇺🇸 Lauren Davis (qualifying competition)

• # 🇺🇸 Alison Riske (qualifying competition)

• # 🇧🇪 Tamaryn Hendler (first round)

• # 🇻🇪 Gabriela Paz (qualifying competition)

• # 🇺🇸 Jennifer Elie (qualified)

Qualifiers
• # 🇫🇷 Aravane Rezai

• # Michelle Larcher de Brito

• # 🇸🇰 Jana Čepelová

• # 🇺🇸 Jennifer Elie