2010 Australian Open – Women's singles

Defending champion Serena Williams defeated Justine Henin in the final, 6–4, 3–6, 6–2 to win the women's singles title at the 2010 Australian Open. It was her fifth Australian Open singles title and twelfth major singles title overall, surpassing the Open Era record (jointly held by Margaret Court, Steffi Graf, Monica Seles and Evonne Goolagong Cawley) for the most Australian Open singles titles. This was Henin's first major since the 2008 Australian Open, following her retirement in May 2008. Henin was unseeded at a major for the first time since the 2001 Australian Open, and was awarded a wild card into the tournament.

Li Na entered the top 10 in the WTA rankings for the first time by reaching the semifinals, becoming the first Chinese player to achieve the feat.

Seeds
• 🇺🇸 Serena Williams (champion)

• 🇷🇺 Dinara Safina (fourth round, retired because of a lower back injury)

• 🇷🇺 Svetlana Kuznetsova (fourth round)

• Caroline Wozniacki (fourth round)

• 🇷🇺 Elena Dementieva (second round)

• 🇺🇸 Venus Williams (quarterfinals)

• 🇧🇾 Victoria Azarenka (quarterfinals)

• 🇷🇸 Jelena Janković (third round)

• 🇷🇺 Vera Zvonareva (fourth round)

• 🇵🇱 Agnieszka Radwańska (third round)

• 🇫🇷 Marion Bartoli (third round)

• 🇮🇹 Flavia Pennetta (second round)

• 🇦🇺 Samantha Stosur (fourth round)

• 🇷🇺 Maria Sharapova (first round)

• 🇧🇪 Kim Clijsters (third round)

• 🇨🇳 Li Na (semifinals)

• 🇮🇹 Francesca Schiavone (fourth round)

• 🇫🇷 Virginie Razzano (first round)

• 🇷🇺 Nadia Petrova (quarterfinals)

• 🇷🇸 Ana Ivanovic (second round)

• 🇩🇪 Sabine Lisicki (second round)

• 🇸🇰 Daniela Hantuchová (third round)

• 🇸🇰 Dominika Cibulková (first round)

• 🇪🇸 María José Martínez Sánchez (second round)

• 🇪🇸 Anabel Medina Garrigues (first round)

• 🇫🇷 Aravane Rezaï (second round)

• 🇷🇺 Alisa Kleybanova (third round)

• 🇷🇺 Elena Vesnina (first round)

• 🇮🇱 Shahar Pe'er (third round)

• 🇺🇦 Kateryna Bondarenko (second round)

• 🇺🇦 Alona Bondarenko (fourth round)

• 🇪🇸 Carla Suárez Navarro (third round) Note: Yanina Wickmayer, who would have been placed in the entry list on the initial entry cutoff date of 7 December 2009 and seeded 16th, entered late and played the qualifying tournament.